| SCET (UTC) | Event |
| 19-01-2006 | Launch |
| 19-01-2006 | Spacecraft separation from carrier rocket |
| 20-01-2006 | Closest approach to the Moon (184,737 km) |
| 20-01-2006 | Passes moon orbit 9 hours after launch. The fastest spacecraft ever to leave Earth (384,000 km) |
| 28-01-2006 | Trajectory Course Maneuver |
| 30-01-2006 | Trajectory Course Maneuver |
| 15-02-2006 | Trajectory Course Maneuver |
| 07-04-2006 | Crosses Mars orbit at a very great distance |
| 13-06-2006 | Asteroid APL (2002JF56) Flyby |
| 24-08-2006 | Pluto downgraded to dwarf planet |
| 08-01-2007 | Image: Jupiter and Io from a long distance away |
| 14-02-2007 | Gravitational tug of Jupiter exceeds that of the Sun. New Horizons enters the Jupiter system. (23,600,000 km) |
| 28-02-2007 | Europa closest approach (2,964,860 km) |
| 28-02-2007 | Jupiter Flyby. Distance 2,304,600 km. Source: SSD |
| 28-02-2007 | Ganymede closest approach (3,017,099 km) |
| 28-02-2007 | Callisto closest approach (4,156,059 km) |
| 28-02-2007 | Io closest approach (2,239,721 km) |
| 15-03-2007 | Gravitational tug of Jupiter is again weaker than that of the Sun. New Horizons leaves the Jupiter system. (24,300,000 km) |
| 02-06-2008 | 10 AU from Sun |
| 07-06-2008 | Closest approach to Asteroid Chiron (11.908 AU) |
| 08-06-2008 | Crosses Saturn Orbit |
| 25-02-2010 | Halfway to Pluto … by distance flown |
| 14-07-2010 | Halfway between the Sun and Pluto (as at C/A) |
| 17-10-2010 | Halfway to Pluto … by time |
| 17-03-2011 | Closest approach to Neptune at 22.988 AU |
| 18-03-2011 | Crosses Uranus Orbit |
| 22-05-2011 | 20 AU from Sun |
| 16-11-2011 | Closest approach to Asteroid Pholus (11.173 AU) |
| 30-07-2014 | 30 AU from Sun |
| 24-08-2014 | Crosses Neptune Orbit |
| 25-08-2014 | 25th anniversary of Voyager 2 Neptune flyby |
| 06-11-2014 | Closest approach to L5 of Neptune at 3.79AU [approximate] |
| 01-05-2015 | Closest approach to Neptune L5 Trojan 2008LC18 (6.005AU) |
| 14-07-2015 | Pluto closest approach (12,500km, 13.78km/s) |
| 14-07-2015 | Charon closest approach (28,858 km) |
| 14-07-2015 | Pluto-Sun occultation |
| 14-07-2015 | Charon-Sun occultation |
| 14-07-2015 | Phone Home – Sent |
| 15-07-2015 | Phone Home – Received |
| 02-12-2015 | KBO 1994 JR1 distant flyby |
| 01-08-2018 | LORRI takes images of galactic deep fields, observing that there may be fewer galaxies in the universe than initially thought. arXiv |
| 01-01-2019 | Arrokoth (2014 MU69) close flyby |